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首页> 外文期刊>Journal of Virology >Late nonstructural 100,000- and 33,000-dalton proteins of adenovirus type 2. I. Subcellular localization during the course of infection.
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Late nonstructural 100,000- and 33,000-dalton proteins of adenovirus type 2. I. Subcellular localization during the course of infection.

机译:腺病毒型的晚期非结构100,000-和33,000dalton蛋白2. I.在感染过程中亚细胞定位。

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摘要

We analyzed the subcellular locations of the late adenovirus type 2 nonstructural 100,000-dalton (100K) and 33K proteins in adenovirus type 2-infected HeLa cells both by biochemical cell fractionation and by immunofluorescence microscopy, using specific antisera against purified sodium dodecyl sulfate-denatured 100K and 33K polypeptides. Both methods showed that the 100K protein was present in the cytoplasm as well as in the nuclei of infected cells and that it accumulated in the nuclei during the course of infection. Phosphorylated 100K protein also was found both in the cytoplasm and in nuclei. However, the nuclear 100K protein pool was phosphorylated to a higher degree than the cytoplasmic pool. In all experiments the 33K protein, which also is a phosphoprotein, was present exclusively in the nuclei of infected cells. The 100K and 33K proteins were associated with different nuclear substructures; this was demonstrated serologically by an analysis of infected cells in which double color immunofluorescence microscopy was used. In these experiments antibodies against the 100K protein decorated different nuclear structures than antibodies against the 33K protein.
机译:通过生物化学细胞分级和免疫荧光显微镜,分析了腺病毒2型非结构100,000-dalton(100k)和33k蛋白的晚期腺病毒2型非结构100,000-dalton(100k)和33k蛋白的亚细胞位置,使用特异性抗血清纯化硫酸钠硫酸钠 - 变性100K和33k多肽。两种方法表明,100K蛋白质存在于细胞质中以及感染细胞的细胞核中,并且它在感染过程中累积在细胞核中。在细胞质和核中也发现磷酸化的100K蛋白。然而,核100k蛋白质池磷酸化比细胞质池更高。在所有实验中,33k蛋白也是磷蛋白的,仅在感染细胞的细胞核中存在。 100K和33K蛋白质与不同的核结构有关;通过对使用双彩免疫荧光显微镜的感染细胞进行血清晶来彻底证明了这一点。在这些实验中,针对100K蛋白的抗体装饰不同的核结构,而不是针对33K蛋白的抗体。

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