Paper-Chromatographic and Paper-Electrophoretic Analysis of Diffusate-Component

摘要

To get a brief picture of Diffusate-component, paper-chromatographic and paper-electrophoretic analysis were done in this experiment. In the fomer analysis natural auxins and free amino acids in Diffusate were detected, in the latter analysis several nucleotide-like substances were skimmed. It is clear that there are two kinds of auxin, and both are neither IAA nor NAA judging from their Rf values and absorption spectra. Aspartic acid, glutamic acid, alanine, valine, leucine, isoleucine and (phenylalanine) were the free amino acids found in Diffusate. Among these amino acids, however, aspartic acid and alanine may be dominating in quantity. Each substance fractionated by paper-electrophoresis has a curve of absorption spectrum of which max. is between 250 and 260 millimicrons.