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Effects of Hard Tissue-Related Hormones on the Intracellular Calcium Ion of the Rat Odontoblasts

机译:硬组织相关激素对成牙本质细胞胞内钙离子的影响

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We examined the effects of PTH, calcitonin (CT) and parotin subunit on the intracellular Ca~2+ of odontoblasts using a chelate reagent, FURA2-AM. Rat CT (rCT), at a final concentration of 0.01 μM, induced a gradual lowering of Ca~2+, and addition of ATP, in the presence of CT, resulted in a partial and short-lasting recovery of Ca~2+. At higher concentrations, it caused a rapid decrease of Ca~2+. CTs of other animal species showed similar effects. Human PTH (hPTH) added at concentrations of 0.01, 0.1 and 1 μm, caused no significant changes in in- tracellular Ca~2+. Parotin subunit caused a rapid lowering of Ca~2+ which was seen already at 0.01 μm.
机译:我们使用螯合剂FURA2-AM检查了PTH,降钙素(CT)和腮腺素亚基对成牙本质细胞内Ca〜2 +的影响。最终浓度为0.01μM的大鼠CT(rCT)诱导Ca〜2 +逐渐降低,并且在存在CT的情况下添加ATP会导致Ca〜2 +的部分和短期恢复。在较高的浓度下,它导致Ca〜2 +迅速降低。其他动物的CT表现出相似的效果。以0.01、0.1和1μm的浓度添加人PTH(hPTH)不会引起细胞内Ca〜2 +的显着变化。帕罗汀亚基导致Ca〜2 +迅速降低,这已经在0.01μm处观察到。

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