Analysis of glycerophospholipid metabolism after exposure to PCB153 in PC12 cells through targeted lipidomics by UHPLC-MS/MS

摘要

Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that have neurotoxicity, reproductive toxicity, hepatotoxicity and immunotoxicity in both animals and humans. Few studies have focused on the changes to endogenous glycerophospholipid metabolism caused by PCB153. To evaluate the relationships between exposure to PCB153 and specific endogenous glycerophospholipid metabolism, an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was implemented in this study. Twenty-two endogenous glycerophospholipids in PC12 cells were analyzed after exposure to PCB153 at dosages of 0.05 mu g mL(-1), 0.5 mu g(-1) or 20 mu g mL(-1) for 120 h. PC(14:0/14:0), PE(16:0/18:1), PE(16:0/18:2), PS(18:0/ 18:1) and PI(16:0/18:1) were identified as potential biomarkers under the rules of t-test (P) value 0.05 and variable importance at projection (VIP) value 1. It was also found that the alterations at 0.05 mu g(-1) and 20 mu g mL(-1) PCB153 were similar at 120 h, while 0.5 mu g mL(-1) PCB153 presented an opposite trend. Additionally, significant upregulation of PC, PE and PS with the same fatty acid chains of 18:0/18:2 was found after exposure to 0.05 mu g mL(-1) and 20 mu g ml(-1) PCB153 at 120 h. This study revealed that PCB153 exposure modulated 22 endogenous glycerophospholipids in PC12 cells and provided the basis for the further study of PCB153 on the effects of glycerophospholipids on PC12 cells.