Effect of Dust on the Viability of Vibrio fischeri in the Microtox Test

摘要

The standard Microtox test involving the bioluminescent bac- terium Vibrio fischeri is a frequently used ecotoxicological bi- oassay whose EC_(50), values have been correlated to acute toxicity parameters of vertebrates, to irritancy measures, and to cytotoxicity indices. The aims were to explore the dependence of light output on viable cell number, with the latter estimated with the naked eye using a colorimetric tetrazolium salt method, the effects of dust on the bioluminescence and cell viability, how the viability of the cells is affected after spills, and how spills can be sampled.