The Relationship Between Humoral and Cellular Immunity to IA-2 in IDDM

摘要

Autoantiboides to the neuroendocrine protein insuli- noma-associated protein 2 (IA-2), a member of the tyrosine phosphatase family, have been observed in individuals with or at increased risk for IDDM. Because this disease is thought to result from a T-cell-mediated autoimmune destruction of the insulin-producing pan- creatic β-cells, we analyzed humoral and cellular immune reactivity to this autoantigen to further define its role in the pathogenesis of IDDM. Peripheral blood mononuclear cells (PBMC) from individuals with newly diagnosed IDDM or at vayring levels of risk for the dis- ease were stimulated in vitro with the entire 42-kDa internal domain of IA-2 (amino acids 603-679), a series of control antigens (glutathionine-S-transferase, tetanus toxoid, Candida albicans, mumps, bovine serum albumin), and a mitogen (phytothemagglutinin).