Glucose Uptake, Utilization, and Signaling in GLUT2-Null Islets

摘要

We previously reported that pancreatic islet β-cells from GLUT2-null mice lost the first phase but pre- served the second phase of glucose-stimulated insulin secretion (GSIS). Furthermore, we showed that the remaining secreotry activity required glucose uptake and metabolism because it can be blocked by inhibi- tion of oxidative phosphorylation. Here, we extend these previous studies by analyzing, in GLUT2-null islets, glu- cose transporter isoforms and glucokinase expression and by measuring glucose usage, GSIS, and glucose- stimulated insulin mRNA biosynthesis.