Changes in the expression of transcription factors in pancreatic AR42J cells during differentiation into insulin-producing cells

摘要

Pancreatic AR42J cells possess exocrine and neuro- endocrine properties and convert to insulin-producing cells upon treatment with activin A and hepatocyte growth factor(HGF). We studied changes in the mRNA expression of various transcription factors during the course of differentiation. Among the transcription fac- tors studied, expression levels of Pax4 and neurogenin3 changed significantly. These two factors were not detected in naive cells, whereas their mRNA levels were markedly increased after treatment with activin A and HGF.