Treatment of type 2 diabetes by adenoviral-mediated overexpression of the glucokinase regulatory protein

摘要

The enzyme glucokinase(GK)plays a central role in glucose homeostasis. Hepatic GK activity is acutely controlled by the action of the GK regulatory protein (GKRP). In vitro evidence suggests that GKRP revers- ibly binds to GK and inhibits its activity; however, less is known about the in vivo function of GKRP. To further explore the physiological role of GKRP in vivo, we used an E1/E2a/E3-deficient adenoviral vector containing the cDNA encoding human GKRP(Av3hGKRP).