Quantification of biodegradable PLGA nanoparticles for drug targeting

摘要

Objective. The aim of this work was the development of appropriateanalytical methods and assays for determining andmonitoring composition and degradation of nanoparticlesbuilt from PLGA (poly D, L-lactid-co-glycolid), which can bereloaded with different drugs. A sensitive and precise methodfor monitoring of nanoparticle degradation in vitro was developedand optimized. Nanoparticles allow a selective enrichmentof different drugs and knowledge of the nature andtype of their degradation is essential for characterization andcontrol of drug release and dosage. Materials and methods. The first method developed during this work to quantify thePLGA polymer matrix use advantage of the chemical reactionof aliphatic carboxylic acids with ferric chloride (FeCl3) thus quantifying both degradation products of PLGA, lacticand glycol acids, at the same time. A second assay method ofchoice was to react to the polymer hydrolysate with lactatedehydrogenase, thus assaying selectively the lactic acid part.Results. During development of both of described methodswas possible to determine dynamic range for PLGA matrixand nanoparticles, as well as to characterize impact of Pluronic F-68 and glycolic acid on lactate dehydrogenase activity?Conclusion. During our work we were able to developtwo sensitive methods for monitoring of biodegradation of polymers which are consecutively used as a nanoparticle matrixin drug targeting.