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首页> 外文期刊>The Journal of biological chemistry >Correction: PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions
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Correction: PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions

机译:校正:PCBP1和PCBP2均结合重氧化RNA,但在氧化条件下导致相反的结果,抑制或增加细胞凋亡

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The following legend has been corrected. In the legend corresponding to Figure 8B, where it reads: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis. ***p < 0.0001 versus all others,###p < 0.0001 versus WT.
机译:纠正了以下传奇。 在对应于图8B的图例中,在其中读取的情况下:B,H2O2暴露后PARP-1的切割。 将细胞(WT,PCBP2-KO,PCBP2-KO + WT和PCBP2-KO + KH1DD)与0.2mM H 2 O 2温育。 在指定的时间,在SDS裂解缓冲液中回收细胞并沸腾。 对样品进行SDS-PAGE,然后使用抗PARP-1抗体(顶部)进行蛋白质印迹。 通过图像量子T1测量印迹中所示的带强度,确定切割PARP1的百分比(底部)。 来自三个独立实验的数据显示为平均值±SE。 双向ANOVA作为统计分析进行。 *** P <0.0001与其他人,### P <0.0001与WT。

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