Bayesian Optimization for Hydrodynamic Characterization of a Cylindrical Orbitally Shaken Bioreactor with a Bump at the Bottom

摘要

An orbitally shaken bioreactor is the most popular system for cell cultivation in a suspension culture owing to its unique characteristics. However, conventional bioreactors struggle to meet the anticipated demand in practical applications due to their limitations. In this study, numerical simulations are conducted to evaluate the performance of a newly devel oped orbitally shaken bioreactor that is equipped with a vaulted “bump” at the bottom wall. The presence of this bump signi cantly improves the mass transfer and cell suspension ratio in the culture medium without increasing the shear stress;it also e ciently reduces the cell aggregation in the central part at the bottom wall. In addition, to enable a suit able cell culture environment for practical applications, the Bayesian algorithm is employed to optimize the control of three parameters, namely bump height (hb), shaking velocity (ω), and shaking radius (R), of this reactor. For all the cases considered, the obtained data related to mass transfer, suspension ratio, and shear stress are analyzed. The simulation results show that the optimal bioreactor is preferable for cell cultivation compared with the initial state, owing to its high capability for mass transfer and suspending cells. It can be concluded that the methodology described in this paper is a feasible and reliable tool for performance prediction and process optimization in biotechnology.