The Effect of Papaya Seeds Extracts Nanoliposomes Administration on Osteoclasts Number of Diabetic Periodontitis Animal Model

摘要

Periodontitis is the most common complication in diabetes mellitus sufferers with a prevalence rate of up to 75%. The previous study proved that papaya seeds extract can inhibit the osteoclastogenesis process. Nevertheless, generally natural compounds have scarce water solubility, and inappropriate molecular size. The modification of the drug delivery system can optimize the therapeutic effect, such as nanoliposome. The research objective is to evaluate the effect of papaya seeds extract nanoliposome on osteoclasts number in the diabetic periodontitis animal model. The study used 36 Sprague Dawley rats which were divided into 3 groups on days 3, 7 and 14. Each group was group K (diabetic periodontitis model mice without treatment), group P1 (diabetic periodontitis model mice given ethanol extract of papaya seeds 96 %) and group P2 (diabetic periodontitis model mice given 96% ethanol extract of papaya seed nanoliposome preparations). Both preparations were given 0.5 ml orally and 0.03 ml dropped on the gingival groove once a day. Osteoclasts were counted by HE staining. The data obtained were tested using One Way Anova and Tukey's Post Hoc test. Based on the results of the One way Anova test on the 14th day, there was a significant difference in the number of osteoclasts (p 0.05) and there were significant differences between groups K and P1 and P2 (p <0.05). The administration of papaya seed extract nanoliposome affects reducing the number of osteoclasts in diabetic periodontitis mice, nevertheless there was no significant difference between the effect of nanoliposome and papaya seeds extract administration.