ANTIOXIDANT ACTIVITY OF MAGNETIZED WATER IN MICE INJECTED WITH Nerium oleander ETHANOLIC EXTRACT

摘要

Exposure of water to a magnetic field increases its solubility, conductivity,and mineral content.Pre-vious studies have shown that magnetized water can cause weight gain, increase milk production, and help treat diseases in livestock. This study aimed to evaluate magnetized water's antioxidant activity in oxidatively stressed mice by Nerium oleander etha-nolic extract.?Forty 6- to 8-week-old Blab/c mice were clas-sified into four groups: control, magnetized water, Nerum oleander, and magnetized water plus Nerium oleander.The weight ofeach mouse was measured at the beginning of the experiment, after 3 and 6 weeks, and at the end of the experiment. After 6 weeks, a blood sample was collected from each mouse in plain and EDTA tubes to measure hemato-logical and chemical parameters, respectively. Moreover, the mice were sacrificed, and then the liver, kidney, and heart were immediately collected and washed with saline and used for measuring oxi-dants and antioxidant parameters and for histopatho-logical examination. In addition to serum ALT, AST, total CK,CK-MB,and c-troponin I levels, the following hematological parameters were measured:Red blood cell(RBC) count,hemoglobin, hemato-crit,and MCV.Moreover,lipid peroxidation (LPO), carbonyl carbon(CC),reduced glutathione(GSH), catalase, glutathione peroxidase(GSH Px), and su-peroxide dismutase(SOD) were measured in he-patic, kidney, and cardiac homogenates.?After 6 weeks and at the end of the experiment, the mice in the magnetized water group had a signif-icantly higher weight gain(p<0.05 and p<0.01, respectively). The hematological parameters did not show statistically significant differences among the groups.Serum AST,ALT,total CK,CK-MB, andc-troponin I levels showed statistically significant dif-ferences between the four groups(p<0.01). In the cardiac homogenate, both LPO and CC levels were significantly reduced in the magnetized water group, while GSH was increased(p<0.01).Moreover, cat-alase,GSH-Px, and SOD activities were signifi-cantly elevated in the magnetized water group in the same homogenate. In the hepatic homogenate, the activity of the antioxidant enzymes catalase, GSH-Px,and SOD was significantly increased in the mag-netized water group (p<0.05). Finally, the activity of catalase was significantly increased in the kidney homogenate of the magnetized water group(p<0.05). The histopathologic appearance of liver and cardiac tissues induced by injection of mice with Ne-rium oleander were milder in group drinking mag-netized water.?The present study concludes that magnetized water increased the activity of the antioxidant en-zymes catalase, glutathione peroxidase, and super-oxide dismutase. These enzymes increased the activ-ity of antioxidant molecules and reduced glutathi-one, while they decreased lipid peroxidation and car-bonyl carbon during oxidative stress in mice. More-over, magnetized water caused a weight gain in these mice, resulting from the increased solubility of this water for minerals and vitamins.