PHYTOCHEMICAL ANALYSIS AND WOUND HEALING ACTIVITY OF CALOTROPIS PROCERA (AIT.) R. BR. LEAVES

摘要

Calotropis procera (Ait) R. Br. (Asclepiadaceae) is a small, hardy, pubescent, evergreen, erect and compact shrub that grows wild in South-Eastern Asia. It is prescribed to treat anasarca, asthma, ascites, bronchitis, cough, cutaneous diseases, intestinal worms, leprosy, and eczema. A methanolic extract of the leaves of Calotropis procera was subjected to chromatography over silica gel column to isolate as six phytoconstituents, and their structures were characterized as n-nonanyl octadec-9-enoate (n-nonanyl oleate, 1), lup-12, 20 (29)-dien-3β-olyl hexadecanoate (lupenyl palmitate, 2), 1-methyl-4-(2′-n-decanyl)-cyclohex-1-en-5-one (3), (Z)-eicos-9-enoic acid (gadoleic acid, 4), n-decanoyl-α-L-arabinopyranoside (n-capryl arabinoside, 5) and stigmast-5- en- 3β- ol- 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside-2′′-hexadecanoate (β-sitosterol-diglucosyl palmitate, 6). The structures of all these natural products have been established on the basis of spectral data analyses for the screening of the wound healing activity of all 6 compounds, and we found Compound-1, 2, 4, and compound 6 having excellent results during wound healing in rats. Our results clearly authenticate the beneficial effects of Compound-1, 2, 4, and 6 for an accelerated wound healing activity.