Clinical efficacy of metagenomic next-generation sequencing for rapid detection of Mycobacterium tuberculosis in smear-negative extrapulmonary specimens in a high tuberculosis burden area

摘要

OBJECTIVE:To assess the clinical utility of mNGS in smear-negative extrapulmonary specimens collected from China.METHODS:Specimens were tested by mNGS and other routine TB tests. The diagnostic accuracy of mNGS was calculated and compared with the final clinical diagnosis.RESULTS:The sensitivity of mNGS is significantly higher than that of other routine TB tests. ROC curves showed mNGS achieved the highest areas under curve (AUC) value of 0.79. The mNGS positive rate of tuberculous meningitis was the highest. All non-tuberculous extrapulmonary pathogens were directly and simultaneously detected.CONCLUSION:The mNGS seemed to be superior to all previous TB etiological tests in smear-negative extrapulmonary specimens and could identify all possible pathogens at once within 48?h.Copyright ? 2020. Published by Elsevier Ltd.