Effect of resveratrol on endocrine function, oocyte oxidation and apoptosis in mice with polycystic ovary syndrome

摘要

Purpose: To investigate the effect of resveratrol on endocrine function, oocyte oxidative stress and oocyte apoptosis in mice with polycystic ovary syndrome (PCOS). Methods: Healthy female Kunming mice (n = 180) were assigned to control, model and resveratrol groups, respectively. Changes in ovarian tissue in each group, including apoptosis of granulosa cells, were measured. Oocyte abnormal mitochondria, changes in oocyte mitochondrial membrane potential, ex pression levels of Bcl-2, Bax and caspase-9 in oocytes evaluated. The activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), and levels of reactive oxygen species (ROS) in each group were also assayed. Results: Compared with the model group, the levels of sex hormones, ovarian cell apoptosis and abnormal mitochondria of mice in the resveratrol group were significantly reduced, while uterine weight increased (p 0.05). The ex pression levels of Bax, Bcl-2, caspase-9, GPx and SOD, and ROS levels in the resveratrol group decreased significantly (p 0.05). Conclusion: Resveratrol significantly improves endocrine function in PCOS mice, but inhibits oocyte apoptosis and oxidative stress response. Thus, resveratrol may be useful in the treatment of PCOS but further clinical trials are required to confirm it.