Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh

摘要

Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC_(50) (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42μg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43μg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.