The inhibiting effects of microRNA-429 on the progression of pancreatic ductal adenocarcinoma cells by inhibiting epithelial mesenchymal transition

摘要

Objective: To research the effects and related mechanism of microRNA (miRNA)-429 in the development of pancreatic ductal adenocarcinoma (PDAC). Methods: The proliferation and invasion ability of cells were evaluated through MTT assay and transwell assay, respectively. The expression of proteins and mRNA were examined by immunofluorescence, western blot, and quantitative real-time polymerase chain reaction (qRT-PCR). Results: The effects and potential mechanism of miR-429 in PDAC cells were explored and evaluated. Our study suggested that miR-429 is closely related with the progression of cancer. Overexpressed miR-429 restricted the mobility and proliferation of PDAC cells by restricting EMT, while down-regulated miR-429 had the opposite effect. These above results implied that miR-429 suppresses the development of PDAC by regulating EMT. Conclusion: MiR-429 inhibits the progression of PDAC cells by regulating EMT. Our study provided a novel potential mechanism for the occurrence of PDAC and laid the foundation for the development of miRNA targeted therapy in patients with PDAC.