Pre-processing tissue specimens with a tissue homogenizer: clinical and microbiological evaluation

摘要

Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize microbiological yield. The objective of this study was to evaluate the added value of using tissue pre-processing (tissue homogenizer instrument gentleMACS Dissociator) in detecting microorganisms responsible for infections. We included 104 randomly collected tissue samples, 41 (39.4?%) bones and 63 (60.6?%) soft tissues, many of those (42/104 (40.4?%)) were of periprosthetic origins. We compared the agreement between pre-processing tissues using tissue homogenizer with routine microbiology diagnostic procedure, and we calculated the performance of these methods when clinical infections were used as reference standard. There was no significant difference between the two methods (McNemar test, p?=?0.3). Among the positive culture using both methods (n?=?62), 61 (98.4?%) showed at least one similar microorganism. Exactly similar microorganisms were found in 42/62 (67.7?%) of the samples. From the included tissues, 55/ 104 (52.9?%) were deemed as infected. We found that the sensitivity of homogenized tissue procedure was lower (83.6?%) than when tissue was processed using tissue homogenizer (89.1?%). Sub-analysis on periprosthetic tissues and soft or bone tissues showed comparable results. The added value of GentleMACS Dissociator tissue homogenizer is limited in comparison to routine tissue processing.