Antioxidant and Anticancer Potential of Raja Bulu Banana Peel and Heart (Musa acuminata Colla (AAB group)) Ethanol Extracts in MCF-7 Cell Lines

摘要

Breast cancer is an uncontrolled cell growth in breast tissue. Surgical treatments of breast cancer can reduce breast aesthetics and chemotherapy can cause severe side effects. It makes the searches for plants as breast anticancer agents intensively carried out. Several studies have shown that banana peels and blossoms possess antioxidant and anticancer activity. This study aims to determine the fruit peel and blossom of Raja Bulu banana (Musa acuminata Colla (AAB group)), an endemic banana species in Indonesia, potential as antioxidant and anticancer agent in MCF-7 cells. Antioxidant potential was determined by using 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay. Anticancer potential was determined by cytotoxic test using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The results showed that 70% ethanol extract of fruit peel (PBEE) and blossom (BBEE) of Raja Bulu banana had median inhibition concentration (IC50) for DPPH scavenging activity at 115.32 μg/mL and 162.52 μg/mL respectively, while for H2O2 scavenging activity at 624.80 μg/mL and 497.13 μg/mL respectively. Anticancer potential was expressed by inhibiting concentration of 50% proliferation (IC50) of MCF-7 cells for PBEE and BBEE were 115.00 μg/mL and 338.47 μg/mL respectively. This study showed that PBEE and BBEE have antioxidant and anticancer properties.