Knockdown of long non?coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA?369?3p/TRIM2 axis

摘要

Idiopathic pulmonary fibrosis (IPF) is the most common form of idiopathic interstitial pneumonia with an increasing incidence. In the present study， Genome Expression Omnibus (GEO) datasets (GSE10667， GSE24206 and GSE32537) were applied to identify lncRNA DLEU2 in IPF. Through prediction using starBase， TargetScan， miRTarBase and miRDB， tripartite motif containing?2?(TRIM2) and prostaglandin F2 receptor inhibitor?(PTGFRN) were found to be upregulated in IPF. DLEU2 expression， the mRNA expression of TRIM2 and PTGFRN， and miR?369?3p expression in A549 cells and lung tissues were detected by RT?qPCR. The protein expression of TRIM2 and PTGFRN in lung tissues and A549 cells was detected by western blot analysis. The proliferation and migration of A549 cells was respectively detected by CCK?8 assay and wound healing assay. The expression of collagen I， α?smooth muscle actin (SMA) and E?cadherin was detected by immunofluorescence assay in A549 cells， and collagen I expression was detected by immunohistochemistry assay in lung tissues. The expression of collagen I， α?SMA and E?cadherin was also detected by western blot analysis in A549 cells and lung tissues.