Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS

摘要

The roots ofO.?fragransare also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents inO.?fragransroots extract was developed, which showed high sensitivity for both qualitative and quantitative analyses. Thirty-two compounds were first discovered inO.?fragransroots, one compound of which was reported for the first time. The simultaneous determination method for acteoside, isoacteoside, oleuropein and phillyrin was validated to be sensitive and accurate. Then it was applied to determine the content of bioactive components inO.?fragransroots from different cultivars. The content of oleuropein and phillyrin in the twelve batches was relatively stable, while the content of acteoside and isoacteoside varied greatly. Moreover, the therapeutic material basis and mechanism ofO.?fragransroots exerting its traditional pharmacodynamics were analyzed by network pharmacology. The results showed thatO.?fragransroots might be effective for the treatment of inflammation, cardiovascular diseases, cancer, and rheumatoid arthritis, which is consistent with the traditional pharmacodynamics ofO.?fragransroots. This work can provide an analytical method for the comprehensive development ofO.?fragransroots.