Flavonoid Profiling Analysis by Liquid Chromatography–Tandem Mass Spectrometry

摘要

Flavonoids are secondary metabolites with antioxidant, anticancer, and anti‐inflammatory properties. However, no method has yet been reported for the simultaneous profiling analysis for flavonoids of various types. Thus, simultaneous profiling analysis of various flavonoids was performed in this study. Under optimal conditions, the developed method showed good linearity (r ≥?0.9973) in the range 25–1000?ng/μL with a limit of detection (LOD) of 0.9–12.1 pg. and a limit of quantification (LOQ) of 2.6–36.5 pg., respectively. The repeatability as a percentage of relative standard deviation (% RSD) varied from 0.5 to 5.9 (% RSD). And accuracy as a percentage of relative error (% RE) was from ?11.8 to 10.5 (% RE). The validated method was applied to a solid phase extraction fraction of Orostachys japonicus (Maxim.) A. Berger. Six flavonoids of catechin, rutin, quercitrin, quercetin, luteolin, and kaempferol were positively identified and quantified. Therefore, the present method will be useful for the monitoring and screening of flavonoids in natural products.