Enantioseparation on Amino Alcohol‐Derived Stationary Phases by Supercritical Fluid Chromatography and High‐Performance Liquid Chromatograpy

摘要

Previous studies have shown that amino alcohol‐derived chiral stationary phases can effectively separate some chiral samples. With the aim at improving the chiral separation, in this study, the composition and type of the mobile phase and the particle size of the stationary phase are changed, and the analytical method is carried out in parallel using high‐performance liquid chromatograpy (HPLC) and supercritical fluid chromatography (SFC). By changing the type and composition of the mobile phase and reducing the particle size of the stationary phase, optimal separation and analysis conditions are obtained simultaneously for HPLC and SFC experiments. In particular, for a particle size of 3 μm, the resolution increases for dinitrobenzoyl phenylglycinol and 2,2,2‐trifluoro‐1‐(9‐anthryl)ethanol. Moreover, replacing the organic modifier from methanol to isopropanol allows obtaining better results in the SFC experiments of dinitrobenzoyl phenylglycinol, dinitrobenzoyl leucinol, and 2,2,2‐trifluoro‐1‐(9‐anthryl)ethanol GTOC,Chiral separation by SFC and HPLC.