Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc

摘要

Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise heating steps during thermal cycling and the measurement of reagent temperature. Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. The experimental results demonstrate that our proposed system provides one-stop detection capabilities for Salmonella with a stable PCR amplification in a single PCR–LOD.