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首页> 外文期刊>RSC Advances >Insights into the production of extracellular polymeric substances of Cupriavidus pauculus 1490 under the stimulation of heavy metal ions
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Insights into the production of extracellular polymeric substances of Cupriavidus pauculus 1490 under the stimulation of heavy metal ions

机译:在重金属离子的刺激下,在刺激下探测Cupriavidus Pouculus 1490细胞外聚合物物质的见解

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Three different methods (a sulfuric acid method, sodium chloride method and vibration method) were used to extract extracellular polymeric substances (EPS) from Cupriavidus pauculus 1490 ( C. pauculus 1490) in the present study. The sodium chloride method was able to extract the maximum amount of EPS (86.15 ± 1.50 mg g ~(?1) -DW), and could ensure minimum cell lysis by detecting glucose-6-phosphate dehydrogenase activity and using scanning electron microscopy. This method was therefore selected as the optimal extraction method and used in subsequent experiments. On this basis, the tolerance of C. pauculus 1490 and variations in EPS secretion after the addition of different metal ions was investigated. The tolerance levels of C. pauculus 1490 to Cd( II ), Ni( II ), Cu( II ) and Co( II ) were 300 mg L ~(?1) , 400 mg L ~(?1) , 400 mg L ~(?1) and 400 mg L ~(?1) , respectively. Low concentrations of these heavy metal ions could promote bacterial growth, while increased concentrations were found to inhibit it. The results show that metal ions, especially Cd( II ), stimulate the secretion of EPS, with an EPS yield reaching 956.12 ± 10.59 mg g ~(?1) -DW at 100 mg L ~(?1) . Real-time polymerase chain reaction (PCR) analysis showed that the key EPS synthetic genes, epsB , epsP and Wzz , were up-regulated. Fourier transform infrared spectroscopy analysis suggested that abundant functional groups in EPS play an important role in heavy metal ion complexation. These results will contribute to our understanding of the tolerance mechanism of microorganisms in the presence of different types and concentrations of metal ions.
机译:三种不同的方法(硫酸法,氯化钠法和振动方法)用于从本研究中从Cupriavidus Pauculus 1490(C.Pauculus 1490)中提取细胞外聚合物物质(EPS)。氯化钠方法能够提取最大的EPS(86.15±1.50mg g〜(α1)-dw),并且可以通过检测葡萄糖-6-磷酸脱氢酶活性并使用扫描电子显微镜来确保最小细胞裂解。因此,选择该方法作为最佳提取方法并用于随后的实验。在此基础上,研究了研究添加不同金属离子后C.烟水1490的耐受性和EPS分泌的变化。 C.PauCucus1490至Cd(II),Ni(II),Cu(II)和Co(II)的耐受性水平为300mg L〜(α1),400mg L〜(α1),400 mg L. 〜(?1)和400mg L〜(?1)。低浓度的这些重金属离子可以促进细菌生长,而发现增加的浓度抑制它。结果表明,金属离子,尤其是Cd(II),刺激EPS的分泌,EPS产率达到956.12±10.59mg g〜(α1)-dw,在100mg l〜(α1)。实时聚合酶链式反应(PCR)分析表明,关键的EPS合成基因,EPSB,EPSP和WZZ,上调。傅里叶变换红外光谱分析表明,EPS中丰富的官能团在重金属离子络合中发挥着重要作用。这些结果将有助于了解在不同类型和浓度的金属离子存在下微生物的耐受机制。

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