NONI (Morinda citrifolia L.) FRUIT EXTRACT POTENTIALLY MAINTAIN THE IMMUNE SYSTEM HOMEOSTASIS OF BALB/C MICE FROM DMBA AND CIGARETTE SMOKES EXPOSURE

摘要

Noni fruit is hereditary used as herbal medicine of various diseases that can arise due to exposure of 7,12-dimethylbenz[a]anthracene and cigarette smoke (DMBA-CS). This research aimed to assess the noni fruit extract (NFE) potential to maintaining the immune system from DMBA-CS exposure. The study with a completely random design was using male BALB/c mice (7-8 weeks old). NFE prepared with aqueous solvent and administered for five weeks (5x/week) with a dose of 30, 90, or 810 mg/kg of body weight (BW) per oral. After five weeks, NFE administration stopped, and mice were exposed with DMBA (20 mg/kg BW per oral) and CS for five minutes in five weeks (2x/week for each). This study also used a healthy group and a group that exposed to DMBA-CS without NFE administration. During the ten weeks of treatment, the body weight was measured. After the whole treatment, liver, kidney, lung, and spleen were isolated and weighed. Splenocyte cells were isolated for Flowcytometry. Data was analyzed in parametric by using One-Way ANOVA and post-hoc Tukey-HSD (p-value 0.05). The results showed the NFE helped the mice to maintain the weight when exposed to DMBA-CS. NFE was prevented the weight loss of the liver but not for spleen, whereas in the kidney and lung did not occur significant weight change in all groups. NFE at certain dosages modulated the expression of TNF-α, IFN-γ, IL-6, and IL-1b. This study indicated that NFE potentially maintains the immune system homeostasis from DMBA-CS exposure.