The allele frequencies of growth hormone gene on the parental and progeny of Ongole-crossbreed cattle population in the North Sulawesi of Indonesia using PCR-RFLP

摘要

The objectives of this research were to identify the allelic frequency of growth hormone?MspI?enzyme-restriction, to define the genetic inequilibrium in selected superior and inferior body weight groups of the cows (G0) and to upgrade genetically the genetic equilibrium of their Ongole-crossbred progenies (G1) population. A total of 74 blood samples were collected from G0 and G1 at the artificial insemination (AI) service center in North Sulawesi Province, Indonesia. Blood samples were screened for the presence of GH?Msp1?enzyme-restriction using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method on 1.2% agarose gel. Data were analyzed using statistical program in Excel XP. The results showed that?Msp1+?allele frequencies in superior and inferior G0 groups were 0.45 and 0.18, respectively. The?Msp1+?allele frequencies of G1 born by superior and inferior G0 groups were 0.50 and 0.21, respectively. These increasing frequencies were contributed byMsp1+?allele inheritance from?Msp1+/+?bull genotype (Krista). Allele frequencies of?Msp1+?in both groups of G0 were not under genetic equilibrium. However, allele frequencies of?Msp1+?in G1 population were under genetic equilibrium. The AI technique should be maintained involving various bull genotypes for breeding purpose of favorable?Msp1+/-?heterozygous genotypes and balancing the genetic equilibrium in progeny population.