In vitro effect of lysophosphatidic acid on proliferation, invasion and migration of human ovarian cancer cells

摘要

Purpose: To evaluate the effect of lysophosphatidic acid (LPA) on the proliferation, invasion and migration ability of 3AO, SKOV3 and CAOV3 human ovarian cancer cell lines. Methods: SKOV3, 3AO and CAOV3 cell lines were respectively treated with LPA. Changes in the proliferation rate of these cell lines were observed after LPA treatment. The cell lines that were not treated with LPA served as control group. Boyden chamber was used to assess cell invasion and migration capability. The ex pression levels of relevant cytokines related to cell migration in the supernatant of CAOV3 cell line were determined using ELISA following LPA stimulation. Results: The cell proliferation rate of human ovarian cancer cell lines was significantly accelerated after in vitro LPA treatment in a concentration-dependent fashion. Boyden chamber assay data indicate that invasion indices in 3AO and CAOV3 cell lines were significantly higher than those in untreated control cell lines (p 0.05). However, no statistical significance was noted between 3AO and CAOV3 cell lines (p 0.05). The ex pression levels of relevant cytokines in the CAOV3 cell line were significantly up-regulated after LPA treatment (p 0.05). Conclusion: LPA intervention in vitro accelerates cell proliferation rate and also significantly up-regulates the ex pression levels of multiple cytokines related to cell migration in human ovarian cancer cell lines, suggesting that LPA plays a significant role in the invasion and migration of SKOV3, 3AO and CAOV3 cell lines.