Toxicity of environmental ozone exposure on mice olfactory bulbs, using Western blot technique

摘要

Environmental ozone (Osub3/sub) exposure has adverse effects on different body systems. This experimental work aimed to study the effect(s) of Osub3/sub exposure on the olfactory bulbs (OB) of Swiss Webster and C57BL/6J mouse strains, using Western blot technique. Both mice strains were exposed to different Osub3/sub doses for different number of exposures and durations. The results indicated that Osub3/sub exposure caused a significant increase in the level of the proteins involved in the oxidative stress state such as 4-hydroxynonenal (4HNE) and Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), in addition to the total OB proteins in Swiss Webster mouse strain. However, this effect was not observed in C57BL/6J mouse strain. Furthermore, CYP1A1 was completely absent in the Green fluorescent protein (GFP) C57BL/6J Osub3/sub exposed mice. Moreover, Osub3/sub exposure caused a significant decrease in the body weight of the tested mice from the two strains.