Analysis of Cell Type–specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag–transgenic Mice

摘要

Casein kinase 1 epsilon is involved in various cellular processes, including cell growth, differentiation, and apoptosis, vesicle transport, and control of the circadian rhythm. Deregulation of CK1? has been linked to neurodegenerative diseases and cancer. To better understand the cell type–specific functions of CK1?, we determined its localization by immunhistochemistry in tissues of healthy, young adult BALB/c mice and in mammary tumors of SV40 T-antigen–transgenic mice. CK1? expression was found to be highly regulated in normal tissues of endodermal, mesodermal, and ectodermal origin and in neoplastic tissue of mammary cancer. The data presented here give an overview of CK1? reactivity in different organs under normal conditions and outline changes in its expression in mammary carcinomas. Our data suggest a cell/organ type–specific function of CK1? and indicate that tumorigenic conversion of mammary glands in SV40 T-antigen–transgenic mice leads to downregulation of CK1?. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.