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Adaptor protein Ruk/CIN85 affects redox balance in breast cancer cells

机译:适配器蛋白ruk / cin85影响乳腺癌细胞中的氧化还原平衡

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Excessive reactive oxygen species (ROS) production may lead to damage of cellular proteins, lipids and DNA, and cause cell death. Our previous findings demonstrated that increased level of adaptor protein Ruk/CIN85 contributes to breast cancer cells malignancy. The aim of this study was to investigate the role of Ruk/CIN85 in the maintaining of the redox balance in cancer cells. Mouse breast adenocarcinoma 4T1 cells with different levels of Ruk/CIN85 expression were used as a model in this study. Activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), aldehyde dehydrogenase (ALDH) and formaldehyde dehydrogenase (FALDH), as well as Hsub2/subOsub2/sub and aldehydes content were measured using fluorometric assays. Gene expression correlations between Ruk/CIN85 and antioxidant enzymes in breast cancer samples were analyzed using ist.medisapiens transcriptomic database. It was demonstrated that Ruk/CIN85-overexpressing 4T1 cells were characterized by increased production of Hsub2/subOsub2/sub and reduced activities of CAT, GPx and SOD. Overexpression of Ruk/CIN85 resulted in decreased content of aldehydes together with increased activity of ALDH, while in Ruk/CIN85-knocked down 4T1 cells, activities of ALDH and FALDH were decreased. The data of transcriptomic analysis revealed the correlations between SH3KBP1 expression and CAT , GPX4 , ALDH1A1 , ALDH1L1 , ALDH2 , GSR , SOD1 in human breast carcinomas samples. The obtained results indicate that adaptor protein Ruk/CIN85 affects redox balance in mouse breast adenocarcinoma 4T1 cells.
机译:过量的反应性氧物种(ROS)生产可能导致细胞蛋白质,脂质和DNA的损伤,并引起细胞死亡。我们以前的研究结果表明,适配器蛋白RUK / CIN85的水平增加有助于乳腺癌细胞恶性肿瘤。本研究的目的是调查Ruk / Cin85在癌细胞中氧化还原平衡中的作用。使用不同水平的Ruk / Cin85表达水平的小鼠乳腺腺癌4T1细胞被用作本研究中的模型。过氧化氢酶(CAT),谷胱甘肽过氧化物酶(GPX),超氧化物歧化酶(SOD),醛脱氢酶(ALDH)和甲醛脱氢酶(FALDH),以及H 2 O 2 和醛含量。使用IST.Medisapiens转录组数据库分析Ruk / Cin85与乳腺癌样品中抗氧化酶之间的基因表达相关性。结果证明Ruk / Cin85过表达4T1细胞的表征通过增加H 2 O 2 和猫,GPX和SOD的活性降低。 Ruk / CIN85的过度表达导致醛含量降低以及ALDH的增加,而在RUK / CIN85敲击4T1细胞中,ALDH和FALDH的活性降低。转录组分析的数据揭示了SH3KBP1表达和CAT,GPX4,ALDH1A1,ALDH1L1,ALDH2,GSR,SOD1在人乳腺癌样品中的相关性。所得结果表明,适配器蛋白RUK / CIN85影响小鼠乳腺腺癌4T1细胞中的氧化还原平衡。

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