Conservation of an Endangered Medicinal Tree Species Taxus wallichian through Callus Induction and Shoot Regeneration

摘要

Callus production and regeneration using leaf and internode as explant was carried outat different combinations of plant growth regulators. Taxu wallichiana leaf explantinitiated significant callus on MS CIM K1 (1.7 mg/l 2, 4-D and 1.0 mg/l Kn) with 4.0 mm 2callus area after 45 days. Similarly MS medium CIM B1 -CIM B4 (1.2, 1.5, 1.7 and 2.0 mg/l ofBAP with 2.0 mg/l of 2,4-D) showed equally significant callus induction but with lesscallus area of 3.0 mm 2 compared to 2,4-D and Kn. For regeneration, shooting wasoptimized with MS SIM IA1 -SIM IA3 (1.5, 2.5, 3.5 mg/l IAA and BAP 2.0 mg/l). Maximumshoot length (1.5 mm) was obtained after 45 days from internode. However, there was noroot formation in the above mentioned combinations.