MICROPROPAGATION OF GINGER (ZINGIBER OFFICINALE VAR. RUBRUM) USING BUDS FROM MICROSHOOTS

摘要

Zingiber officinale var. Rubrum (ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle.The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surfaceof the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplementedwith BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant(3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokininwere used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effectivein promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS mediumsupplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAPenhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshootsmaintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and rootsper explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).