RESPONSE OF RICE POLYPHENOL OXIDASE PROMOTER TO DROUGHT AND SALT STRESS

摘要

Polyphenol oxidases (PPO) widely exist in plants that catalyze oxygen dependent oxidation of phenols to quinines andassumed to be involved in plant defense against environmental stresses. In this study transgenic T1 seeds of Arabidopsisthaliana containing Oryza sativa Polyphenol oxidase (OsPPO) gene promoter fused with GUS (β-glucuronidase) wereanalyzed for drought and salt stress. These seeds were already available in our research group. Seeds were germinated onMurashige and Skoog (MS) mediato get T2 plants which were screened in drought stress (5, 10, 15, 20, 25 and 30% PEG6000)and salt concentrations (50 mM, 100 mM, 150 mM, 200 mM, 250 mM and 300 mM). Experimental data showed thatrelative GUS expression of OsPPO gene promoter increased with the increase of drought stress. In case of salt stress,OsPPO induction showed similar trend GUS expression was increased. The response of OsPPO to drought and salt stresssuggest the possible participation of PPO in plants defense against drought as well as salt stress.