Cloning and functional analysis of low nitrogen tolerance related gene CsMYB21 in cucumber

摘要

To investigate the molecular mechanism of low nitrogen (N) tolerance in cucumber, a low N tolerance related gene, named CsMYB21 was cloned in the present study. qRT-PCR showed that CsMYB21 expression was significantly induced under low N condition. Phylogenetic analysis showed that the homology of the gene with the melon was the highest. Functional analysis results showed that root lengths and fresh weights of Col-0 plants were significantly reduced under low N condition, while reduction ratio of root lengths and fresh weights in transgenic line harboring 35S::CsMYB21 was less than Col-0, which revealed that transgenic Arabidopsis plants showed higher capacity for low N tolerance than Col-0. These results revealed that the cucumber CsMYB21 could improve low N tolerance in transgenic Arabidopsis plants.