Phytochemical profiling and pharmacological evaluation of Ifloga spicata (Forssk.) Sch. Bip. in leishmaniasis, lungs cancer and oxidative stress

摘要

Ifloga spicata ( I. spicata ), is an ethno-medicinally important plant. Its crude extract (Is.Cr) and subsequent fractions: n -hexane (Is.Hex), chloroform (Is. Chf), ethyl acetate (Is.EtAc) and aqueous (Is.Aq) were evaluated for potential applications in leishmaniasis, lungs cancer and oxidative stress. Total phenolic content (TPC), Total flavonoid contents (TFC), Total Antioxidant Capacity (TAC) were determined colorimetrically. Samples were screened for antileishmanial effect using Leishmania tropica promastigotes, for cytotoxicity using NCI-H460 cell lines, and for antioxidant potentials via DPPH, ABTS assays. In the phytochemical analysis Is.EtAc exhibited highest amount of TPCs (141.01±0.12μg GAE/mg DW) and TFC of (52.75±1.22μg QE/mg DW). Is.Cr, Is.Hex, Is.Chf, Is.EtAc and Is.Aq demonstrated antileishmanial effect with IC 50 values of 184, 573, 212, 52, 483 μg/ml respectively. Is.Chf showed prominent cytotoxic effect (IC 50 79± 2.9μg/mL) against lung cancer cell line. In DPPH and ABTS assays, Is.EtAc was most potent with IC 50 of 30.44 and 31.79 μg/ml respectively. These findings indicate that I. spicata crude extract and its derived fractions are safe sources of natural antioxidant, cytotoxic and antileishmanial compounds and thus need further studies.