LEAF PROTEOME ANALYSIS OF CLEMATIS CHINENSIS: A TRADITIONAL CHINESE MEDICINE (TCM) BY TWO-DIMENSIONAL

摘要

Leaf proteome of Clematis chinensis, a traditional Chinese medicine (TCM) was analyzed by two-dimensionalelectrophoresis (2-DE) technique. The samples were extracted by phenol-SDS method (PSM) with high protein quantity i.e.2.35±0.345 mg/g (yield/dw). Proteins were visualized by staining of gels by silver stain and CBB. The gel images of eachspecies were compared by Image Master 2D Platinum software for analytical purpose. The 2-DE profile depicteddistribution of 1085 spots and out of these only 255 protein spots (23.5%) were common to all analyzed taxa. The visualizedprotein spots showed pI range from 3.0 to 10.0 (pH) and Mr of 7 kDa to 70 kDa. Twelve proteins were exclusively specificto C. chinensis when compared with its allies, C. finetiana and C. armandii, which may be used as biomarkers. Thirteenproteins were up-regulated in C. finetiana (0.75-0.95 fold) and twelve proteins in C. armandii (1.05-1.66 fold) whilst sevenproteins down-regulated (0.66-0.94 fold) in former and three proteins (1.07-1.20 fold) in later one in comparison with C.chinensis. Twenty five differential and similar protein spots were picked and analyzed by LC-MS/MS technique. Identifiedproteins are related to energy metabolism (ATP synthesis), photosynthesis, environmental stimuli, regulating RNAmetabolism, growth hormone regulators, evolutionary trends and gene expression. The efficiency and applicability ofproteomic approach as biomarker for identification of C. chinensis is discussed in its quality control (QC) perspectives. Leafproteins of Clematis plants are explored for the first time by 2-DE technique and debated for their metabolic role.