TAXONOMIC RELATIONSHIPS OF SOME SPECIES OF OROBANCHE L. EVIDENCE FROM RAPD-PCR AND ISSR MARKERS

摘要

The taxonomic relationships among 25 samples representing nine species of Orobanche L. (Orobanchaceae) weredetermined by the analysis of morphological characters and molecular polymorphism using random amplified polymorphicDNA (RAPD) and inter simple sequence repeat (ISSR). In order to construct dendrogram elucidating the relationshipsamong the examined taxa, the coded data were analyzed using the software package NTSYS-pc 2.1 based on the Neighborjoining(NJ) tree building method based on a distance matrix. The aim of this study is to develop taxonomic relationshipbased on morphological and molecular data, in order to obtain a more reliable taxonomic relationship of Orobanche speciesunder study. The dendrogram produced by the analysis of the molecular data (RAPD and ISSR) resembled that constructedby NJ dendrogram for the morphological variation. The studied taxa were separated in two groups, the first comprised ofthe five species of section Trionychon (O. purpurea, O.lavandulacea, O. ramosa, O. mutelii and O. aegyptiaca) and thesecond comprised of the four species of section Orobanche (O.cernua, O. crenata, O. minor and O. pubescens). Highsimilarity was detected between O. pubescens and O. minor. The results confirmed the close relationship between O.ramosa and O. mutelii. Moreover, this study demonstrated the grouping of the studied taxa in most cases by geographicallyisolated population.