SERINE/THREONINE PHOSPHATASE TAPP2Cs MIGHT BE SERVED AS AN EARLY SIGNAL MOLECULE FOR WATER STRESS IN WHEAT

摘要

Efficient tissue culture system is important for transformation of important genes in hybrid tomato cultivars. Thepresent study was undertaken to develop an efficient tissue culture system for hybrid tomato cultivar Peto-86. The youngprimary leaves and stems were inoculated into five different MS media having different concentrations of plant growthregulators in different combinations for callus induction, somatic embryogenesis and for both direct and indirectregeneration. Maximum callus induction frequency 90% was achieved with MS media containing 2,4-D 4 mg L-1 and BAP0.5 mg L-1. The direct somatic embryogenesis was found highest on MS media supplemented with 2,4-D 4 mg L-1 and BAP0.5 mg L-1. Maximum indirect regeneration frequency 87% was achieved from primary leaves explants with MS mediacontaining IAA 0.5 mg L-1 and BAP 3 mg L-1 and highest direct regeneration frequency 77% was obtained from primaryleaves explants with MS media containing NAA 1 mg L-1 and BAP 3 mg L-1. The high concentration of 2,4-D increasedcallus induction and somatic embryogenesis frequencies while the high concentration of BAP increased regenerationfrequency. An improved tissue culture system of hybrid tomato cultivar Peto-86 was established and it may berecommended for further transformation experiments.