EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES, FRUIT AND BARK OF KIGELIA AFRICANA

摘要

In vitro antibacterial activity of extracts was tested against six bacterial strains viz. Staphylococcus aureus, Proteusvulgaris, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Citrobacter amalonaticus by agar-discdiffusion method. Ethanol and n-hexane were used as negative control and oxytetracycline was used as a positive control.Ethanolic and aqueous extracts of bark and leaves of Kigelia africana showed remarkable activity against various bacterialstrains as compared to n-hexane. S. aureus and E. coli were proved as highly sensitive strains while K. pneumonia was theresistant strain as the extracts formed no inhibition zone against it. The percentage of antioxidant activity of different partsof Kigelia was assessed by DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical assay. Quercetin was used as a standardantioxidant which showed 93.6% inhibition. Kigelia bark extract showed good antioxidant activity i.e., 67.33% inhibition,fruit extract possess moderate antioxidant activity i.e., 62.66% inhibition while leaves showed the poor antioxidant activityi.e., 59.66% DPPH inhibition respectively. Overall, the comparative analysis revealed that bark extract exhibited the mostremarkable antibacterial as well as antioxidant activity as compared to leaves and fruit extracts.