Intestinal Histological Evaluation and Antidiarrhoeal Effect of Allium sativum Juice in Experimentally Induced Diarrhoea in Rats

摘要

Background and Objective: Diarrhea is one of the leading causes of mortality in less developed countries yearly. Hence, it has become pertinent in modern medical research to search for new sources of antidiarrhoeal drugs. The present study evaluated the effects of Allium sativum cloves juice extract on lactose monohydrate and castor oil-induced diarrhoea in albino rats. Materials and Methods: Antidiarrhoeal effect of Allium sativum juice extract (ASJE) was evaluated using lactose monohydrate/castor oil-induced diarrhoea and gastrointestinal motility tests using two doses (5 mL and 10 mL kgsup?/supsup1/sup b.wt.) of ASJE. Body weight changes and faecal droppings were recorded and the caeca of rats were excised for necropsy and histological studies. The activity of ASJE was compared to loperamide (5 mg kgsup?/supsup1/sup) and atropine sulphate (3 mg kgsup?/supsup1/sup) which were used as standard reference drugs. Results: ASJE showed a marked antidiarrhoeal effect by inhibiting 69.23 and 76.92% of diarrhoea at doses of 5 and 10 mL kgsup?/supsup1/sup, respectively. The extract also significantly reduced intestinal transit (p0.05) in GI motility test compared to the corresponding control. Caecum distention, goblet cell hyperplasia and inflammatory cellular were observed to be markedly prevented by ASJE treatment. The effects observed were comparable to those of the standard drugs. Conclusion: The findings from the present study indicated that the juice extract of Allium sativum cloves possess the ability to prevent experimentally induced diarrhoea in rats.