Determining the Biofilm Forming Gene Profile of Staphylococcus aureus Clinical Isolates via Multiplex Colony PCR Method

摘要

Background:Among hospitalized patients, Staphylococcus aureus (S. aureus) infections pose a serious healththreat. The present study investigated the frequency of biofilm forming genes among clinical isolates S. aureusand their susceptibility to antibiotics.Methods: The clinical samples were analyzed via standard biochemical assays for identifying differentbacterium, which was then confirmed using the multiplex colony PCR method. Those samples identified as S.aureus were examined for the presence of the cna, fnbA, fnbB and pvl genes. The antibiotic susceptibility of theS. aureusisolates was then tested.Results:Using the standard biochemical assay approach, 54 S. aureus strains were identified. However, whenusing the multiplex PCR method 50 S. aureus strains were identified among the clinical samples. The in vitrobiofilm formation assays determined 3 (6%) strains of S. aureus to be strong biofilm forming, 15 (30%) of theisolates were determined to be moderate biofilm forming and, 32 (64%) were determined to be weak biofilmforming. Among the isolated strains, the specific frequencies of the biofilm forming genes were determined tobe 31 (62%) for cna, 35 (70%) for fnbA, 13 (26%) for fnbB and 1 (2%) for pvl. In 11 (22%) of the isolatedstrains fnbA, fnbB and cna genes were all present. All strains were determined to be penicillin, amoxicillin andclavulanic acid resistant.Conclusions: Due to the increase of the antibiotic resistance in biofilm producing S. aureus strains, rapididentification of antibiotic resistance can help to eliminate the infection effectively.