Role of calbindin-D28K in estrogen treatment for Parkinson's disease

摘要

Studies have shown that estrogen has neuroprotective effects on the nigrostriatal system. The present study established a Parkinson's disease model in C57BL/6 mice by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrapyridine. The mice were subjected to 17β estradiol injection into the lateral ventricle. Immunofluorescence double staining showed that estrogen increased tyrosine hydroxylase and calbindin-D28K expression and co-expression in dopaminergic neurons of midbrain substantia nigra pars compacta of model mice. Behavior experiments showed that estrogen improved swimming and hanging behaviors in this mouse model of Parkinson's disease. Research Highlights (1) Estrogen can protect dopaminergic neurons from 1-methyl-4-phenyl-1,2,3,6-tetrapyridine-induced injury in the brain. (2) The neuroprotective effects were achieved by upregulating the calcium-binding protein expression pathway.