MINERALIZATION KINETICS OF HYDROXYAPATITE/CHITOSAN COMPOSITE IN A SIMULATED BODY FLUID

摘要

The objective of this study is to investigate on real time the mineralization process of hydroxyap- atite/chitosan (HAP/CS) composite in a simulated body fluid using the piezoelectric quartz crystal impedance (PQCI) technique and electrochemical impedance spectroscopy (EIS) to reveal its kinetics processes for bone tissue engineering. The morphology and composition for the corresponding stages of mineralization products were characterized by Fourier transformed infrared (FT-IR), X-ray diffraction (XRD) and scanning electrode microscopy (SEM). A decrease of the frequency (f) and an increase of the charge transfer resistance (_^ct) at the gold elec- trode/solution interface were observed due to the mineralization of HAP/CS composite. According to the data of the frequency and static capacity (Cs), the correlative kinetic equations and parameters were obtained by nonlinear regression. The results show that the mineralization process of HAP/CS is divided into two stages: the nucleation stage and the crystal growth stage. The experiments show that the proposed method could provide real time and multidimensional information to the monitoring of biomaterial mineralization process.