INHIBITORY AND SYNERGISTIC EFFECT OF NOVEL COMBINED ACTIVE SUBSTANCES OF FUNGICIDES AGAINST SELECTED PLANT PATHOGENS

摘要

We determined minimal inhibitory concentration (MIC) of single active substances (a.s) of fungicides, twelve mixtures of two a.s and four a.s mixture. Additionally fractional inhibitory concentration index (FICI) of combined a.s. was calculated. Fungal and oomycete pathogens included Fusarium culmorum, Colletotrichum coccodes, Penicillium verrucosum, Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternata, Aspergillus candidus, Monilinia fructicola, Septoria tritici and oomycete Phytophthora infestans. MIC were ranged from 0.1 pg/ml for fluazinam to 4954.5 pg/ml for cymoxanil. MIC of combined a.s were ranged from 0.00004 pg/ml for picoxystrobin+fenamidone to 4955 pg/ml for cy- moxanil+carboxin. Dodine+picoxystrobin had shown the most effective inhibitory and synergistic action against seven fungal or oomycete species (0.001 pg/ml - 35.9 pg/ml). The highest decrease of MIC values of dodine+picoxystrobin compared to their separate application indicate that these compounds should be considered to create novel commercial formulations of fungicides.