Inosine Released from Dying or Dead Cells Stimulates Cell Proliferation via Adenosine Receptors

摘要

Figure 1. Stimulation of tumor cell proliferation by bystander dying cells, the “feeder cells” effect. The upper panel shows viable cell counts of cocultures [closed circles in panels (A–C)] and viable cells alone in D10 medium [open circles in panels (A–C)]. Cocultures were composed of viable (200 cells/well) and lethally ultraviolet light type B-irradiated (10,000 cells/well) B16F10 melanoma cells (A). Dead/viable ratio titration of cocultures (B). Transwell cocultures with 10,000 apoptotic cells (C). The lower panel shows cell counts of independent wells containing fibroblasts in R10 medium [open circles in panels (D–F)] or in supernatants (SN) from apoptotic cells [closed circles in panels (D–F)]. Mouse NIH/3T3 fibroblast (1,000 cells/well) cultures with SNs of 0.2 million cells/ml apoptotic homologous cells (D). Human fibroblast-like synoviocytes (FLS, 1,000 cells/well) from two rheumatoid arthritis patients RA315 (E) and RA314 (F) cocultured with SNs from apoptotic neutrophils (0.2 million cells/ml).