SCREENING OF PHYTOCHEMICALS, ANTIOXIDANT AND INHIBITORY EFFECT ON ALPHA-AMYLASE BY ETHANOLIC EXTRACT OF ELAEOCARPUS GANITRUS (BARK)

摘要

Elaeocarpus ganitrus Roxb is widely used in traditional medicine to cure various diseases. In this present study, the phytochemical constituents, antioxidant activity and inhibitory effect on alpha amylase by the ethanolic extracts of the Elaeocarpus ganitrus (bark) were investigated. The dried bark of Elaeocarpus ganitrus was extracted by using ethanol as a solvent for extraction with the help of Soxhlet apparatus. The solvent free crude was screened for presence of phytochemicals, antioxidant activity and alpha amylase inhibitory effect. The screening for phytochemicals showed the presence of carbohydrates, alkaloids, steroids, flavonoids, phenol, tannin, Saponin, glycosides and terpenoids. The ethanolic extract showed a concentration dependent increase in antioxidant activity (Upto 79% by DPPH method and upto 72.8% by H₂O₂ method), which can be used as a potential anti-aging agent and in the treatmentof free radical associated diseases. The ethanolic extract also showed a dose dependent increase in inhibitory effect on alpha amylase (Upto 82%) which can be used as a potential source as antidiabetic drug. From the current study it can be seen that the bark extract of Elaeocarpus ganitrus contain diversity of phytochemicals, it is also a potential antioxidant agent and it showed a strong alpha amylase inhibitory activity which may be used as a natural anti-diabetic agent.