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Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species

机译:Tamarix Gallica的十七个新微卫星和Tamarix物种中的交叉放大

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Premise Microsatellite markers were developed for the western Mediterranean tree Tamarix gallica (Tamaricaceae) as part of a study of its genetic diversity and structure. Methods and Results Seventeen microsatellite markers were developed for T. gallica , 14 of which were polymorphic. These microsatellites have di‐, tri‐, and tetranucleotide repeats with 1–13 alleles per locus and population. Levels of observed and expected heterozygosity ranged from 0.000 to 0.900 and from 0.000 to 0.863, respectively. Six microsatellites showed significant deviations from Hardy–Weinberg equilibrium in at least one population. Cross‐amplification in 19 Tamarix species showed a wide transferability to other species of the genus. Conclusions The 14 new polymorphic microsatellite markers will be used to assess the genetic diversity and population genetic structure of T. gallica . Additionally, the successful cross‐species amplification suggests their potential usefulness for investigating species delimitation and population genetics in the genus Tamarix .
机译:作为研究其遗传多样性和结构的研究的一部分,为西地中海树Tamarix Gallica(Tamaricaceae)开发了前提微卫星标记。方法和结果为17种微卫星标志物用于Glotica,其中14个是多态的。这些微卫星具有二核,三核苷酸的二核苷酸,每个基因座和群体的1-13等位基因。观察和预期杂合子的水平分别为0.000至0.900和0.000至0.863。六间微露露透露术表现出至少一种群体中的Hardy-Weinberg平衡的显着偏差。 19种Tamarix物种中的交叉扩增表现出对该属的其他物种的广泛可转化性。结论将使用14种新的多晶型微卫星标志物评估T. Gallica的遗传多样性和群体遗传结构。此外,成功的交叉物种扩增表明它们在塔米松属中调查物种划界和人口遗传学的潜在有用性。

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